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| Production and dynamic of destruxins from Metarhizium spp. |
| Xu Jinzhu,Qin Changsheng,Yang Hua,Zhao Danyang,Jie Yuze,Qiu Hualong |
| (Guangdong Provincial Key Laboratory of Silviculture, Protection
and Utilization/Guangdong Academy of Forestry, Guangzhou,) |
| Abstract: |
| Taking the yield of DxtA and DxtB as index-
es, the difference of the destruxin producing ability of
15 Metarhizium spp. strains (belonged to 5 species) was
analyzed. The DxtA dynamic curve of the best strain
was fitted under shaking flask culture by sampling every
day. The results showed that there was a significant dif-
ference in the destruxin producing ability among 5 gen-
era. Metarhizium anisopliae strains showed the highest
production, M. lepidiotae produced a little Dxts, while
M. flavoviride, M. taii and M. acridum did not produce
DxtA or DxtB. Among them, the M19 strain was the
best to produce DxtA (144.12 μg/mL), and the M25
strain was the best to produce DxtB (65.31 μg/mL). The
M25 was the best in DxtA and DxtB production. Under
shaking flask culture, the DxtA production of M25 was
fitted by SPSS, and showed to fit a Logist model（Y
=84.99/(1+11.82×e -0.3X ). In the first four days, the M25
was in vegetative growth period and produced a little
Dxts. From the fifth day, it changed into exponential
growth and reached a plateau from the ninth day. |
| Key words: Meterhizium · Destruxin · Dynamics curve |
